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Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody(Cat. #P32636). P32636 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#P32636) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#P32636). P32636 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Mouse Monoclonal Antibody to CAPN2
-
货号：
P32636 -
别名：
Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2 -
应用：
WB,IHC-P,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P17655 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to CAPN2
Description |
|---|
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. |
Specification |
|
|---|---|
| Aliases | Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2 |
| Entrez GeneID | 824 |
| Swissprot | P17655 |
| WB Predicted band size | 80.0kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/100 |
Product Image
- Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody(Cat. #P32636). P32636 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
- Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#P32636) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
- Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#P32636). P32636 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32636
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