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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using SNAI2 mouse mAb against MCF-7 cell lysate.
Immunofluorescence analysis of Hela cells using SNAI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of MCF-7 cells using SNAI2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using SNAI2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using SNAI2 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to SNAI2
-
货号：
30730 -
别名：
SLUG; WS2D; SLUGH1; SNAIL2 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O43623 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to SNAI2
Description |
|---|
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. |
References |
|---|
| 1. Cancer Med. 2013 Apr;2(2):144-54. 2. FEBS J. 2012 Aug;279(16):2929-39. |
Specification |
|
|---|---|
| Aliases | SLUG; WS2D; SLUGH1; SNAIL2 |
| Entrez GeneID | 6591 |
| Swissprot | O43623 |
| clone | 4B6D5 |
| WB Predicted band size | 30kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SNAI2 (AA: 100-200) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using SNAI2 mouse mAb against MCF-7 cell lysate.
- Immunofluorescence analysis of Hela cells using SNAI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of MCF-7 cells using SNAI2 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using SNAI2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using SNAI2 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30730
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