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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using B4GALNT1 mouse mAb against Hela (1), Jurkat (2), HepG2 (3), k562 (4), COS-7 (5), and NIH/3T3 (6) cell lysate.
Flow cytometric analysis of Hela cells using B4GALNT1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human brain tissues using B4GALNT1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using B4GALNT1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to B4GALNT1
-
货号：
32224 -
别名：
GALGT; SPG26; GALNACT; GalNAc-T -
应用：
WB,IHC,FCM -
反应种属：
Human, Mouse, Monkey -
抗体类型：
Primary antibody -
Swissprot：
Q00973 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to B4GALNT1
Description |
|---|
GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. |
References |
|---|
| 1,Exp Mol Pathol. 2019 Aug;109:25-35. 2,Sci Rep. 2020 Jan 27;10(1):1199. |
Specification |
|
|---|---|
| Aliases | GALGT; SPG26; GALNACT; GalNAc-T |
| Entrez GeneID | 2583 |
| Swissprot | Q00973 |
| clone | 4G11B1 |
| WB Predicted band size | 58.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Monkey |
| Immunogen | Purified recombinant fragment of human B4GALNT1 (AA: 26-225) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using B4GALNT1 mouse mAb against Hela (1), Jurkat (2), HepG2 (3), k562 (4), COS-7 (5), and NIH/3T3 (6) cell lysate.
- Flow cytometric analysis of Hela cells using B4GALNT1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human brain tissues using B4GALNT1 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using B4GALNT1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32224
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