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Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to SMAD5
-
货号：
30150 -
别名：
Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q99717 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to SMAD5
Description |
|---|
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous. |
References |
|---|
| 1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32. 2. Nat Cell Biol. 2008 May;10(5):567-74. |
Specification |
|
|---|---|
| Aliases | Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 |
| Entrez GeneID | 4090 |
| Swissprot | Q99717 |
| clone | 3H9 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
- Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).
- Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30150
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