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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Western blot analysis using PRKAA1 mouse mAb against COS7(1),C2C12(2),NIH/3T3(3) cell lysate.
Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to PRKAA1
-
货号：
30172 -
别名：
AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Mouse,Rat,Monkey -
抗体类型：
Primary antibody -
Swissprot：
Q13131 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PRKAA1
Description |
|---|
The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
References |
|---|
| 1. Oncol Rep. 2008 Dec;20(6):1553-9. 2. Placenta. 2008 Dec;29(12):1003-8. |
Specification |
|
|---|---|
| Aliases | AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1 |
| Entrez GeneID | 5562 |
| Swissprot | Q13131 |
| clone | 2B7 |
| WB Predicted band size | 64kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PRKAA1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
- Western blot analysis using PRKAA1 mouse mAb against COS7(1),C2C12(2),NIH/3T3(3) cell lysate.
- Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30172
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