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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human prostate tissues using PRK2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PRK2 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to PRK2
-
货号：
30252 -
别名：
PKN2; PAK2; PRK2; Pak-2; PRKCL2; PRO2042; MGC71074; MGC150606 -
应用：
WB,IHC,FCM -
反应种属：
Human,Mouse,Rat,Monkey -
抗体类型：
Primary antibody -
Swissprot：
Q16513 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PRK2
Description |
|---|
Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis. |
References |
|---|
| 1. Cell. 2009 Jul 23;138(2):389-403. 2. Ann Intern Med. 2009 Apr 21;150(8):541-50. |
Specification |
|
|---|---|
| Aliases | PKN2; PAK2; PRK2; Pak-2; PRKCL2; PRO2042; MGC71074; MGC150606 |
| Entrez GeneID | 5586 |
| Swissprot | Q16513 |
| clone | 1D1 |
| WB Predicted band size | 140kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PRK2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
- Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human prostate tissues using PRK2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PRK2 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30252
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